Nucleic Acid Amplification Test Tuberculosis

The health system benefits and cost effectiveness of using mycobacterium tuberculosis direct nucleic acid amplification testing to diagnose tuberculosis disease in the united states.

Nucleic acid amplification test tuberculosis. Since then naa testing has become a routine procedure in many institutions for the diagnosis of tb because naa tests can rapidly and reliably detect mycobacterium tuberculosis bacteria directly in a specimen one or more weeks earlier than. Nucleic acid amplification testing may be helpful in determining whether patients who have tested positive for acid fast bacilli have tuberculosis according to the findings of a new study. Diagnosis of pulmonary tuberculosis using pcr assays on sputum collected. It was co developed by the laboratory of professor david alland at the university of medicine and dentistry.

The genexpert mtb rif cepheid sunnyvale ca is an automated molecular test for detection of m. Nucleic acid amplification naa tests such as polymerase chain reaction pcr and other methods for amplifying dna and rna may facilitate rapid detection of microorganisms. Tuberculosis in clinical specimens. Nucleic acid amplification tests naats offer another technique for the direct detection of m.

Traditional methods for laboratory diagnosis of tuberculosis tb may require weeks and delay can impede treatment and control efforts. The xpert mtb rif is a cartridge based nucleic acid amplification test naat for simultaneous rapid tuberculosis diagnosis and rapid antibiotic sensitivity test it is an automated diagnostic test that can identify mycobacterium tuberculosis mtb dna and resistance to rifampicin rif. Tuberculosis with sensitivity and specificity that approaches culture. Nucleic acid amplification tests for tuberculosis.

Nats differ from other tests in that they detect genetic materials rna or dna rather than antigens or antibodies. Rather the negative naa test result should be used as additional information to aid in making clinical decisions to expedite a work up for an alternative diagnosis or to prevent unnecessary use of tb treatment in suspects with a. 23 the test is simple to perform and gives results in 100 minutes. A nucleic acid test nat is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of organism often a virus or bacteria that acts as a pathogen in blood tissue urine etc.