Nucleic Acid Amplification Test Dengue

Nucleic acid extraction amplification and amplicon detection.

Nucleic acid amplification test dengue. Amplification of dengue viral rna. The first step is to extract nucleic acid sample and its purification. Final nucleic acid extracts were obtained in a total volume of 50 μl of elution buffer. Test view normal values test results.

Summary of operating characteristics and comparative costs of dengue diagnostic methods. A naat is a generic term referring to molecular tests used to detect viral genomic material. Tissue tests for dengue virus may be performed on biopsy or autopsy specimens. Second stage involves amplification of that nucleic acid and then detection along followed by characterization of the amplified product.

A naat like real time reverse transcription polymerase chain reaction rrt pcr or in situ hybridization detects dengue virus rna. The current study describes the development of an internally controlled multiplex nucleic acid amplification test for the detection of dengue virus denv and leptospira and plasmodium species with a specific callout for plasmodium falciparum referred to as the ufi assay. This special controls document was developed to support the classification into class ii special controls of dengue den virus denv nucleic acid amplification test naat reagents. How should it be used and at what time during.

Dengue virus nucleic acid amplification test reagents are devices that consist of primers probes enzymes and controls for the amplification and detection of dengue virusserotypes 1 2 3 or 4. Among these steps nucleic acid extraction is the first step of sample preparation which remains one of the main challenges when converting laboratory molecular assays into poc tests. Nucleic acid amplification test naat what is the test. Nucleic acid amplification test naat for fixed tissue specimens.

This procedure utilized 100 μl of plasma or serum as the starting input material. What is the test. Detection of viral rna in plasma or serum or tissues using nucleic acid amplification test naat. Nucleic acid was extracted from dengue virus stocks and from human serum or plasma test samples using the method of boom et al.

A 3 confirmed dengue a suspect or probable dengue case with positive result of viral culture and or polymerase chain reaction pcr and or nucleic acid amplification test loop mediated amplification assay naat lamp and or plaque reduction neutralization test prnt b. Dengue rna pcr qual. A typical nucleic acid based diagnostic test consists of three major steps.